Figure illustrates the temporal distinction between humans, rats, and zebrash in early developmental life. During development, there are still several limitations in the chemical identication of zebrash.The analysis of gene expression throughout the larvae is feasible due to the optical transparency of the zebrash tissues, which enables good penetration for light microscopy. An alternative preliminary toxicity test is called the brine shrimp lethality test, which involves testing dierent toxicant concentrations.However, this technique does not reveal abnormalities or causes of death. In contrast, using zebrash as a screening model animal has some rational benets, including large embryos, large numbers of embryos for testing, and simple visualization of organogenesis using reasch Encequidar uorescence and transgenic strains. Additionally, the ability to view biological processes provides the researcher with visual links to the organ system dynamics and, possibly, interorgan systems. Today, many herbal products claim to provide pharmaceutical health benets but do not provide any toxicological data; thus, the safety of these drugs is questionable.This review aimed to compare the results of studies of the toxicity of medicinal plants and assess the toxicity tests used to analyze some of these commercial products from plants.For instance, recent studies of acute toxicity have mainly used mice as the animal model.Nevertheless, it is dicult to achieve a thorough and immediate toxicity check with this organism because of its strong state of breeding, high expenses, complicated procedures, and ethical restrictions. Instead of using rodents, sh are the best candidate for this purpose.Fish traditionally have been used in toxicity testing of individual substances and euents, and today zebrash are commonly utilized to test for developmental toxicity, general toxicity and to carry out medication screening as a credible vertebrate model. Hence, the rst largescale studies for bioactive molecules using zebrash embryos were published, using the merits of zebrash as phenotypic testing models to evaluate the eects of biomolecules and explore dierent bioactive compounds. Zebrash embryos can also quickly consume tiny molecular compounds, thus providing a valuable model for drug testing and evaluating teratogenic eects. Zebrash embryos and larvae are outstanding models for testing the toxicity of substances, especially if those substances are present in low quantities. Zebrash larvae are very useful in imaging studies, and they likely will prove useful for nonimaging endpoints.Scientists are discovering new innovative paths for evaluating biochemical processes. Teratology is the study of unusual growth, and a teratogen is any substance that triggers the production of a congenital anomaly or enhances the occurrence of a specic hereditary deciency. Screening for teratogenicity includes introducing zebrash embryos to the required concentration of the compound of interest, and it has become a popular model for analyzing the teratogenic eects of medicines. Teratological and embryotoxic eects are easy to detect due to the transparency of zebrash embryos during their buy Metyrosine growth outside of their parent. Teratogenic eects include tail malformation, pericardial edema, malformation of the notochord, scoliosis, yolk edema, and growth delays. Therefore, it is essential to evaluate the embryotoxic and teratogenic toxicity of medicinal plants on embryo growth.The eectiveness of the zebrash model system was evident in the s and s, as numerous studies used zebrash as bioassays of chemicals aecting normal functioning and reproductive success.